A water soluble carbodiimide also called EDAC or EDCI, commonly obtained as the hydrochloride. Typically, it is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided. It can be used as a chemical crosslinker for collagen, reacting with the carboxylic acid groups of the collagen polymer which can then bond to the amino group in the reaction mixture.1,2 Efficiency of EDC-mediated coupling is increased in the presence of Sulfo-NHS.3 Excess reagent and crosslinking byproducts are easily removed by washing with water or dilute acid. Crosslinking can be done in physiologic solutions without adding organic solvent. Applications for EDC include conjugate carboxyl to amine groups in peptides and proteins;4,5 convert carboxyls to amine-reactive Sulfo-NHS esters;6,7 crosslink proteins to carboxyl coated beads or surfaces;8 Activate nanoparticles with amine-reactive Sulfo-NHS esters;9 couple haptens to carrier proteins (e.g. attach a peptide to KLH);10 DNA labeling through 5’ phosphate groups.11
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