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Capping A, 20% NMI in ACN, for AKTA oligopilot™ (Acetonitrile / N-methylimidazole, v/v = 80:20) 1L, 2.5L, GL45 thread emp Biotech GmbH

CAS Number:
Molecular Formula:
Molecular Weight: 0.00
MDL Number:

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Regular Price: $99.00
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In Stock USA
Regular Price: $165.00
Your Discounted Price: $0.00
In Stock USA

Signal Word:Danger
Hazard Statements:H225, H302, H312, H314, H332
Precautionary Statements:P201, P261, P280, P301 + P310, P301 + P330 + P331, P302 + P350, P304 + P340, P305 + P351 + P338, P310
Packing Group:II
Hazard Class:3
, 8

Limited Quantities:1.0 L (0.3 gallon) (liquid)
Excepted Quantities:Max Inner Pack (30g or 30ml) and Max Outer Pack (500g or 500ml)

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For each synthesis cycle, up to 1 to 2 % of free 5'-hydroxy groups remain after the phosphoramidite coupling step has been completed. By running a subsequent "Capping" step using an anhydride, these free hydroxyl groups are converted to acetates and are hindered from further chain elongation and formation of long oligonucleotides with incorrect sequences. For optimal acetylation, a solution of acetic anhydride in Tetrahydrofuran or acetonitrile (Capping A) will be mixed in situ during reaction with a catalytic acting solution of N-methylimidazole (Capping B). Additives such as pyridine and lutidine function as mild bases to enhance the efficiency of the capping reaction.

Product of emp Biotech GmbH
Size bottle:
1L with GL45 thread, emp Biotech part no. NC-0705-N001.0-001
2.5L with GL45 thread, emp Biotech part no. NC-0705-N002.5-001

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