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Visit Us:Marm 2024 Celebrating Discovery

June 5th-June 8th, 2024

The Pennsylvania State University

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106218

106218-450ml



Capping B, 16 % NMI in THF, emp Biotech GmbH (THF/N-methylimidazole, V / V = 84 : 16) NC-0801, 450ml : 28-400 thread, 2.5L : GL45 thread


CAS Number: 616-47-7
   
Molecular Formula: C4H6O3
   
Molecular Weight: 102.09
   
MDL Number:


 Item #PriceQuantityAdd to Cart 
106218-450ml
Regular Price: $60.00
Your Discounted Price: $0.00
In Stock USA
106218-2.5L
Regular Price: $165.00
Your Discounted Price: $0.00
In Stock USA



Bp:64°
 
Pictograms:
Signal Word:danger
Hazard Statements:H225, H302, H312, H314, H318, H335, H336, H351, EUH018, EUH019
Precautionary Statements:P201, P202, P210, P231, P233, P235 + P410, P240, P241, P242, P243, P261, P280, P301 + P310, P301 + P330 + P331, P302 + P350, P303 + P361 + P353, P304 + P340, P305 + P351 + P338, P310, P403 + P233, P403 + P235
UN#:UN2924
Packing Group:II
Hazard Class:3
, 8

SDS: See MSDS
 
Limited Quantities:1.0 L (0.3 gallon) (liquid)
Excepted Quantities:Max Inner Pack (30g or 30ml) and Max Outer Pack (500g or 500ml)

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For each synthesis cycle, up to 1 to 2 % of free 5-hydroxy groups remain after the phosphoramidite coupling step has been completed. By running a subsequent "Capping" step using an anhydride, these free hydroxyl groups are converted to acetates and are hindered from further chain elongation and formation of long oligonucleotides with incorrect sequences. For optimal acetylation, a solution of acetic anhydride in Tetrahydrofuran or acetonitrile (Capping A) will be mixed in situ during reaction with a catalytic acting solution of N-methylimidazole (Capping B). Additives such as pyridine and lutidine function as mild bases to enhance the efficiency of the capping reaction.

Prepacked in two bottle sizes:
450 mL in a 28-400 thread glass bottle, emp Biotech part no. NC-0801-M450.0-001
2.5L in a GL45 thread glass bottle, emp Biotech part no. NC-0801-N002.5-001

Product of emp Biotech

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