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Visit Us:Marm 2024 Celebrating Discovery

June 5th-June 8th, 2024

The Pennsylvania State University

University Park, PA

 

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106219

106219-450ml



Capping B (10 % N-methylimidazole in THF / pyridine, V / V = 80 : 10) NC-0803 emp Biotech GmbH


CAS Number: 616-47-7
   
Molecular Formula: C4H6N2
   
Molecular Weight: 82.11
   
MDL Number:


 Item #PriceQuantityAdd to Cart 
106219-450ml
Regular Price: $60.00
Your Discounted Price: $0.00
In Stock USA
106219-2.5L
Regular Price: $210.00
Your Discounted Price: $0.00
In Stock USA



 
Pictograms:
Signal Word:Danger
Hazard Statements:H225, H302, H313, H314, H318, H335, H336, EUH018, EUH019
Precautionary Statements:P201, P202, P210, P231, P233, P235 + P410, P240, P241, P242, P243, P261, P280, P301 + P310, P301 + P330 + P331, P302 + P350, P303 + P361 + P353, P304 + P340, P305 + P351 + P338, P310, P403 + P233, P403 + P235
UN#:UN2924
Packing Group:II
Hazard Class:3
, 8

Flash Point:-14°
SDS: See MSDS
 
Limited Quantities:1.0 L (0.3 gallon) (liquid)
Excepted Quantities:Max Inner Pack (30g or 30ml) and Max Outer Pack (500g or 500ml)

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For each synthesis cycle, up to 1 to 2 % of free 5'-hydroxy groups remain after the phosphoramidite coupling step has been completed. By running a subsequent "Capping" step using an anhydride, these free hydroxyl groups are converted to acetates and are hindered from further chain elongation and formation of long oligonucleotides with incorrect sequences. For optimal acetylation, a solution of acetic anhydride in Tetrahydrofuran or acetonitrile (Capping A) will be mixed in situ during reaction with a catalytic acting solution of N-methylimidazole (Capping B). Additives such as pyridine and lutidine function as mild bases to enhance the efficiency of the capping reaction.

Prepacked in
450mL, 28-400 thread glass bottle, emp Biotech part no. NC-0803-M450.0-001
2.5 L with GL45 thread glass bottle, emp Biotech part no. NC-0803-N002.5-001

Product of emp Biotech

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