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Capping B2 (60 % lutidine in acetonitrile, for AKTA oligopilot™) NC-0807, GL45 thread emp Biotech GmbH

CAS Number: 108-48-5
Molecular Formula: C7H9N
Molecular Weight: 107.16
MDL Number:

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Regular Price: $90.00
Your Discounted Price: $0.00
In Stock USA
Regular Price: $135.00
Your Discounted Price: $0.00
In Stock USA

Signal Word:Danger
Hazard Statements:H225, H315, H319, H335
Precautionary Statements:P210, P233, P280, P305 + P351 + P338
Packing Group:II
Hazard Class:3
Flash Point:10°
Limited Quantities:1.0 L (0.3 gallon) (liquid)
Excepted Quantities:Max Inner Pack (30g or 30ml) and Max Outer Pack (500g or 500ml)

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For each synthesis cycle, up to 1 to 2 % of free 5'-hydroxy groups remain after the phosphoramidite coupling step has been completed. By running a subsequent "Capping" step using an anhydride, these free hydroxyl groups are converted to acetates and are hindered from further chain elongation and formation of long oligonucleotides with incorrect sequences. For optimal acetylation, a solution of acetic anhydride in Tetrahydrofuran or acetonitrile (Capping A) will be mixed in situ during reaction with a catalytic acting solution of N-methylimidazole (Capping B). Additives such as pyridine and lutidine function as mild bases to enhance the efficiency of the capping reaction.

Product of emp Biotech GmbH

Size bottle:
500 mL with GL45 thread, emp Biotech part no. NC-0807-M500.0-001
1.25L with GL45 thread, emp Biotech part no. NC-0807-N011.2-001

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